Variations in genomic epidemiology and in-silico screening of potential phytochemicals to cure Monkeypox

Monkeypox virus (MPXV) is passed on when people encounter infectious animals. Before April 2022, the Monkeypox virus was reported only in South Africa and its surrounding region but now it has been spread all over the world. This Monkeypox virus consumes an incubation period of five to twenty-one days and can be communicated through direct contact, breathing, contaminated towels, bedding, and so on. The Orthopoxvirus variety is a subfamily of the Poxviridae family that incorporates the Monkeypox infection. Their unique property is to suppress the host defense system and to exploit host immunity. Treatment of Monkeypox involves two vaccines named JYNNEOSTM and ACAM2000. Antiviral medications can be considered for serious diseases, immunocompromised patients, pediatrics, pregnant and lactating ladies, complex sores, and when injuries happen close to the mouth, eyes, and privates. This review article gives a basic information ofA48R, a thymidine kinase, which is involved in DNA replication pathways in the Monkeypox virus. The potential drugs for A48R inhibition like NMCT and rutaecarpine are considered good synthetic drugs. The maximum affinity -18 was shown by phytochemical dictamnine, amentoflavone -7.5, citral -7.8, and naringin – 6.6 which can be isolated from different plants.  The purpose of this review article is to describe variations in genomic epidemiology and in-silico screening of potential phytochemicals to cure Monkeypox.Keywords: Monkeypox virus; Orthopoxvirus; Poxviridae; A48R; Phytochemicals

Impact Of Job Status And Demographic Trends On Quality Education: A Case Study Of Private Universities In Pakistan

The major purpose of this paper was to examine the quality of various aspects of higher education in the private sector of Pakistan by adopting a descriptive method of research. The population of the study constituted 270 administrators, 6,180 teachers and 61,108 students in existing 54 private universities and degree-awarding institutions of Pakistan. The method of cluster sampling was used to select the study sample of 840 people, which was carried out in two stages. In the first stage, 12 clusters of universities were randomly chosen out of the total population of private universities. In the second stage, 60 administrators, 180 teachers and 600 students were selected through a random sampling procedure with five administrators, 15 teachers and 50 students from each selected cluster. Three questionnaires, developed and refined through pre-testing, were used as measuring instruments to collect data. The researcher personally visited each university and collected data from the sample. The collected data was tabulated, analyzed and interpreted by using ANOVA and t test techniques

Navigating Research Enthusiasm in Medical Students Towards Clinically Impactful Articles

Learning medical research is an integral part of the development of a holistic physician. Though, not all physicians become clinician-scientists, those who do become enjoy significant privileges over their clinician-only counterparts, mainly in terms of faster career progression and higher employability. Though, to produce more well-rounded clinician-scientists, academic physicians and medical scientists need to make themselves available and be willing to work with and mentor medical students. Besides, a curriculum reform is warranted where students should be encouraged to start learning and conducting research in their first year of medical school. To make the process even easier and help scale these ideas, students should be encouraged to replicate previous highly cited studies, as they can provide a walkthrough for students to follow, thereby necessitating lesser supervision while maintaining the clinical impact that can be made with their time and effort

The Assessment and Mapping of Urban Visual Pollution through an Assembly of Open Source Geospatial Tools

Urban surroundings and spaces are losing their identity due to the visual pollution in the urban panorama of already densely populated cities in the developing countries. Quantitative assessment of visual pollution and its spatial mapping are very recent and relatively un-explored branches of urban studies. The diversity of visual pollution objects (VPOs) and their traits, the subjectivity of observers, the scale of urban space and dependency on subjective variables are the key challenges for quantification during visual pollution assessment (VPA). A paper-based score-card type VPA tool using Analytical Hierarchy Process to addressthese issues has previously been developed. However, considering the challenges associated with the deployment of paper-based tool for VPA (which include the inability to handle variety of data types such as text, numeric, geolocation, images, etc.), the natural progression is the development of a mobile-based solution which matches the fast-growing mobile penetration rate of urban centres and provides a turn-key solution to achieve efficiency and effectiveness in primary data collection. On the other hand, academic research on the spatial mapping of visual pollution has slightly progressed to explore its cartographic dimension. This research presents a spatial decision support system comprising of a combination of open source tools to collect, store and present VPA data for any urban space of any scale. The system employs Open Data Kit (ODK) to build its mobile-based VPA tool which can be used to collect VPO attributes using any Android device. The collected data is streamed to a web-based data management module of the systemin real time which is built upon ODK Aggregate and PostgreSQL. Furthermore, the web-based visualisation module of the system is built upon some other major open source tools including OpenGeo Suite and PHP.The visualisation module presents the results of visual pollution index (VPI) in the form of a web-based dashboard containing real-time choropleth maps which can be filtered for any specific VPO. This research demonstrates the strengths of open geospatial tools to solve challenges of primary data collection on a diverse range of VPOs along with the systematic capturing of their spatial location and visual images. Furthermore, it proves the ability of open source web mapping tools to display visual pollution assessments in most appropriate cartographic representation. Resultantly, it offers the practitioner urban planners a tested mechanism to assess and map the levels of urban visual pollution in an urban space and help them take effective measures to improve the visual image of the city

Comparison of Outcome of two different Methods for the treatment of Intra-articular fracture of Distal Radius

Objective: It is to compare the radiological and functional outcome of two different fixation methods for displaced intra articular distal radius fracture (DRF): open reduction and internal fixation with anatomical plate and closed reduction with percutaneous K-wires. Methodology: A randomized controlled trial was conducted in Orthopaedics department, Shaikh Zayed Hospital, Lahore for one year; from April 2017 to March, 2018. Total 90 patients were selected in the study by ‘non-probability, consecutive sampling technique’ with 45 patients in each group of the study; Group-A (fixation method: Open reduction with internal fixation with volar plate) and Group-B (Fixation method: Kirschner-wire known as K-wire). Results: The mean age in the group A and group B was 53.7 ± 11.8 years and 55.2 ± 12.3 years respectively. There were 58 male and 32 females in the study. The differences in volar tilt, radial inclination, radial length and modified Mayo score were significantly better in group A than group B (p-value < 0.05). The differences were not significantly different for articular step off in the two study groups (p-value > 0.05). Conclusion: Open reduction with internal fixation using volar locking plates yielded significantly better radiological and functional outcomes than percutaneous fixation using K-wire in the 6 months’ postoperative period

Mitigating Urban Visual Pollution through a Multistakeholder Spatial Decision Support System to Optimize Locational Potential of Billboards

Urban visual pollution is increasingly affecting the built-up areas of the rapidly urbanizing planet. Outdoor advertisements are the key visual pollution objects affecting the visual pollution index and revenue generation potential of a place. Current practices of uninformed and uncontrolled outdoor advertising (especially billboards) impairs effective control of visual pollution in developing countries. Improving this can result in over 20% reduction of visual pollution. This article presents a spatial decision support system (SDSS) to facilitate all the stakeholders (development control authorities, advertisers, billboard owners, and the public) in balancing the optimal positioning of billboards under the governing regulations. In terms of its technical implementation, SDSS is based on well-known geospatial open source technologies and uses an analytical hierarchy process AHP-inspired approach in spatial decision-making. It can help users through its category-specific user interface to identify potential sites to position new billboards and the selection of boards from existing sites based on a wide variety of characteristics. The observations of all stakeholders have been recorded through panel feedback to assess the system’s initial effectiveness. The proposed system has been found functional in identifying hot spots for the focused management and exploration of the best suitable sites for new billboards. So, it helps the advertising agencies, urban authorities, and city councils in better planning and management of existing billboard locations to optimize revenue and improve urban aesthetics. The system can be replicated in other countries irrespective of spatial boundaries by incorporating jurisdictional rules and regulations

Potential application of nanotechnology in the treatment, diagnosis, and prevention of schistosomiasis

Schistosomiasis is one of the neglected tropical diseases that affect millions of people worldwide. Globally, it affects economically poor countries, typically due to a lack of proper sanitation systems, and poor hygiene conditions. Currently, no vaccine is available against schistosomiasis, and the preferred treatment is chemotherapy with the use of praziquantel. It is a common anti-schistosomal drug used against all known species of Schistosoma. To date, current treatment primarily the drug praziquantel has not been effective in treating Schistosoma species in their early stages. The drug of choice offers low bioavailability, water solubility, and fast metabolism. Globally drug resistance has been documented due to overuse of praziquantel, Parasite mutations, poor treatment compliance, co-infection with other strains of parasites, and overall parasitic load. The existing diagnostic methods have very little acceptability and are not readily applied for quick diagnosis. This review aims to summarize the use of nanotechnology in the treatment, diagnosis, and prevention. It also explored safe and effective substitute approaches against parasitosis. At this stage, various nanomaterials are being used in drug delivery systems, diagnostic kits, and vaccine production. Nanotechnology is one of the modern and innovative methods to treat and diagnose several human diseases, particularly those caused by parasite infections. Herein we highlight the current advancement and application of nanotechnological approaches regarding the treatment, diagnosis, and prevention of schistosomiasis